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Fluorescent measurements

• Fluorometry

• Ratiometric imaging

• Fluorescent cytometry

 

Model systems

• 2D and 3D mammalian cell culture

• Primary tissue and cells

• Mus musculus

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New tools

• Protein engineering

• Rational design

• fluorescence screening

• directed evolution/selection

 

Examples of techniques used in lab

• Cell and tissue culture

• Protein expression

• Molecular biology and DNA cloning

• Protein purifications

• Viral transduction

•  qPCR

• Western blotting

• High-resolution imaging

• Mammalian cell screening

• Kinetics/enzymology